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Image Search Results


Nuclei from quiescent (Q) or proliferating MBs (MB) and non-reactivated myotubes (MT) were incubated in XEE in parallel. Thirty minutes before times 1, 3, 5, 7, and 9 h, one incubation mixture per nucleus type was pulse-labeled with Cy3-dCTP for 30 min before times 1, 3, 5, 7, and 9. The graph shows the percentages of Cy3-positive nuclei at each time point. Continuous Cy3 labeling throughout the experiment of an identical reaction set (three reactions: Q, MB, and MT) showed that all nucleus types were reactivated similarly: at the 5 h time point, Cy3-positive nuclei were: Q, 96%; MB, 95%; MT, 93%.

Journal: EMBO Reports

Article Title: Structural obstruction to full DNA replication in terminally differentiated skeletal muscle cells

doi: 10.1038/s44319-025-00554-x

Figure Lengend Snippet: Nuclei from quiescent (Q) or proliferating MBs (MB) and non-reactivated myotubes (MT) were incubated in XEE in parallel. Thirty minutes before times 1, 3, 5, 7, and 9 h, one incubation mixture per nucleus type was pulse-labeled with Cy3-dCTP for 30 min before times 1, 3, 5, 7, and 9. The graph shows the percentages of Cy3-positive nuclei at each time point. Continuous Cy3 labeling throughout the experiment of an identical reaction set (three reactions: Q, MB, and MT) showed that all nucleus types were reactivated similarly: at the 5 h time point, Cy3-positive nuclei were: Q, 96%; MB, 95%; MT, 93%.

Article Snippet: Cy3-dCTP , GE Healthcare , PA53021.

Techniques: Incubation, Labeling